cercopithecus aethiops kidney normal cell line  (ATCC)

Journal: Nutrients

Article Title: Macrofungal Extracts as a Source of Bioactive Compounds for Cosmetical Anti-Aging Therapy: A Comprehensive Review

doi: 10.3390/nu16162810

Figure Lengend Snippet: Overview of research on the anti-aging effects of mushroom extracts on skin.

Article Snippet: , in vitro Mus musculus skin melanoma cell line (B16-F10 ATCC, CRL-6475) and Cercopithecus aethiops kidney normal cell line (Vero ATCC, CCL-81); mouse fibroblast cell line (L929) , The multifunctional peptide derived from Ganoderma lucidum was sequenced via LC-MS/MS as NH2-PVRSSNCA-CO2H (octapeptide). Its antioxidant activity was evaluated at 1 mg/mL using DPPH, ABTS and FRAP assays. The octapeptide demonstrated antioxidant capacities of 0.121 ± 0.01 mg ascorbic acid equivalent, 0.173 ± 0.03 mg gallic acid equivalent and 2.21 ± 0.23 mM FeSO 4 equivalent, comparable to established antioxidants. Proteomics analysis identified 5804 proteins and highlighted several pathways affected by the octapeptide in melanoma cells. Targeted proteomics revealed that pigmentation-related proteins were upregulated, while Tyrosinase-Related Protein 1 was downregulated in the treated group. The octapeptide, at concentrations from 1.5625 μg/mL to 100 μg/mL, did not cause significant cell death in either melanoma or Vero cells. , [ ] .

Techniques: In Vitro, Expressing, Functional Assay, Modification, Concentration Assay, Inhibition, Antioxidant Activity Assay, In Vivo, Extraction, Ethanol Precipitation, Emulsion, Activity Assay, Purification, Molecular Weight, Mouse Assay, FRAP Assay, Positive Control, Isolation, Irradiation, Activation Assay, Protein-Protein interactions, Derivative Assay, Targeted Proteomics, In Silico, Cell Cycle Assay, Phospho-proteomics, Produced, Control, Generated, Cream, Formulation, Immunohistochemical staining, Sonication, Negative Control, Diffusion-based Assay, Zeta Potential Analyzer